کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8271711 | 1534977 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparative analysis of ER stress response into HIV protease inhibitors: Lopinavir but not darunavir induces potent ER stress response via ROS/JNK pathway
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CARTSQVIDVFDAIndinavirNFVRTVTPVLPVDRVATVAPVN-acetyl-l-cysteinePRIJnkNACPBMCROS - ROSAmprenavir - آمپرنواویرAtazanavir - اتازناویرER stress - استرس استAIDS - ایدزCHOP - تکه کردنDarunavir - دارنویورcombination antiretroviral therapy - درمان ضد رتروویروسی ترکیبیRitonavir - ریتونویرFood and Drug Administration - سازمان غذا و داروSaquinavir - ساکویناورperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Lopinavir - لوپیناویرProtease inhibitor - مهارکننده پروتئازHIV protease inhibitor - مهارکننده پروتئاز HIVNelfinavir - نلفینوایرARE - هستندAntioxidant response - پاسخ آنتی اکسیدانPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparative analysis of ER stress response into HIV protease inhibitors: Lopinavir but not darunavir induces potent ER stress response via ROS/JNK pathway Comparative analysis of ER stress response into HIV protease inhibitors: Lopinavir but not darunavir induces potent ER stress response via ROS/JNK pathway](/preview/png/8271711.png)
چکیده انگلیسی
HIV protease inhibitor (PI)-induced ER stress has been associated with adverse effects. Although it is a serious clinical problem for HIV/AIDS patients, comparative analyses of ER stress induction by clinically used PIs have rarely been done. Especially, there is no report on the differential ER stress response between lopinavir (LPV) and darunavir (DRV), although these PIs are the most clinically used PIs. We show here that LPV induces the most potent CHOP expression, ER stress marker, among the 9 Food and Drug Administration (FDA)-approved PIs in human peripheral blood mononuclear cells, several human epithelial cells, and mouse embryonic fibroblasts. LPV induced the most potent ROS production and JNK activation in 9 PIs. A comparison among the most clinically used PIs, ritonavir (RTV), LPV, and DRV, revealed that LPV potently and RTV moderately but not DRV induced ER stress via ROS-dependent JNK activation rather than proteasome inhibition. Finally, we analyzed ER stress induction in tissues of mice intraperitoneally injected with RTV, LPV, and DRV. RTV and LPV but not DRV showed ER stress induction in several mice tissues. In conclusion, we first identify LPV as the most potent ER stress inducing PI among 9 FDA-approved PIs in human cells, and although clinical verification is necessary, we show here that DRV has the advantage of less ROS and ER stress induction potential compared with LPV in vitro and in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 65, December 2013, Pages 778-788
Journal: Free Radical Biology and Medicine - Volume 65, December 2013, Pages 778-788
نویسندگان
Manabu Taura, Ryusho Kariya, Eriko Kudo, Hiroki Goto, Takao Iwawaki, Masayuki Amano, Mary Ann Suico, Hirofumi Kai, Hiroaki Mitsuya, Seiji Okada,