Age related depth profiles of human Stratum Corneum barrier-related molecular parameters by confocal Raman microscopy in vivo

The presented results let suggest, that the decreased trans-epidermal water loss (TEWL) with increasing age cannot be sufficiently explained by only the increased SC thickness, but additionally by the increase of ICL ordering, higher NMF concentration and thus larger amount of hydrogen bound water molecules at the depth 20-40% of the SC thickness.