کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8284700 1535582 2018 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Laminin-332 regulates differentiation of human interfollicular epidermal stem cells
ترجمه فارسی عنوان
لامینین 332 سلول های بنیادی اپیدرمال بین فضایی انسان را تنظیم می کند
کلمات کلیدی
سلول بنیادی اپیدرمال اینترفلالیکولار، لامینین 332، تفکیک، طاقچه سلول بنیادی،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
چکیده انگلیسی
Interfollicular epidermal stem cells (IFE-SCs) have self-renewal and differentiation potentials, and maintain epidermal homeostasis. Stem cells in vivo are regulated by the surrounding environment called niche to function properly, however, IFE-SC niche components are not fully understood. In order to elucidate the mechanisms of keeping epidermal homeostasis and of skin aging, and also to develop new therapeutic technologies for skin diseases, we searched for niche factors that regulate IFE-SCs. We found that laminin-332, a basement membrane component, was highly expressed at the tips of the dermal papillae, where IFE-SCs are localized, and that the stem cells by themselves expressed laminin-332. Knockdown of laminin-332 during the culture of IFE-SC-model cells to construct 3-dimensional epidermis in vitro resulted in failure to form proper structure, although no significant change was observed in either cell growth or apoptosis. Pre-coating of the culture insert with laminin-332 restored the normal formation of 3-dimensional epidermis. From these results, it was shown that laminin-332 is an essential niche component for the proper differentiation of IFE-SCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 171, April 2018, Pages 37-46
نویسندگان
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