کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8288485 | 1536252 | 2018 | 41 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dibutyryl cAMP- or Interleukin-6-induced astrocytic differentiation enhances mannose binding lectin (MBL)-associated serine protease (MASP)-1/3 expression in C6 glioma cells
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کلمات کلیدی
pKaAstrocytic differentiationLectin complement pathwayMASPLCPdbcAMPGBMSTAT3GAPDHGFAPDMEMIL-6Dulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccointerleukin-6 (IL-6) - اینترلوکین 6 (IL-6)interleukin-6 - اینترلوکین ۶CNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیsignal transducer and activator of transcription 3 - مبدل سیگنال و فعال کننده رونویسی 3Glial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالGlial fibrillary acidic protein (GFAP) - پروتئین اسیدی فیبریلاگر گلایال (GFAP)protein kinase A - پروتئین کیناز Aglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازGlioblastoma multiforme - گلیوبلاستوم مولتیفرم، گلیوبلاستوما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Mannose-binding lectin (MBL)-Associated Serine Proteases (MASP)-1 and 3, key enzymes in the lectin complement pathway of innate immune response, are also expressed in glioma cell lines. We investigated MASP-1 and MASP-3 expression during dibutyryl cyclic AMP (dbcAMP)- or Interleukin-6 (rIL-6)-induced astrocytic differentiation of C6 glioma cells. Our results demonstrate that C6 cells express basal levels of MASP-1 and MASP-3 and following exposure to dbcAMP or IL-6, a consistent MASP-1 and MASP-3 mRNA up-regulation was found, with a behavior similar to that showed by the fibrillary acidic protein (GFAP). Furthermore, in cell conditioned media, rIL-6 stimulated MASP-3 secretion which reached levels similar to those obtained by dbcAMP treatment. Moreover, the detection of a 46-kDa MASP-3 suggested its processing to the mature form in the extracellular cell medium. Interestingly, the H89 PKA inhibitor, mostly affected dbcAMP-induced MASP-1 and MASP-3 mRNA levels, compared to that of rIL-6, suggesting that cAMP/PKA pathway contributes to MASP-1 and MASP-3 up-regulation. MASP-1 and MASP-3 expression increase was concomitant with dbcAMP- or rIL-6-induced phosphorylation of STAT3. Our findings suggest that the increase in intracellular cAMP concentration or rIL-6 stimulation can play a role in innate immunity enhancing MASP-1 and MASP-3 expression level in C6 glioma cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 653, 1 September 2018, Pages 39-49
Journal: Archives of Biochemistry and Biophysics - Volume 653, 1 September 2018, Pages 39-49
نویسندگان
Valentina Pagliara, Maddalena Parafati, Annagrazia Adornetto, Misti C. White, Mariorosario Masullo, Maurizio Grimaldi, Rosaria Arcone,