کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8289571 1536321 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Computational strategies for the design of new enzymatic functions
ترجمه فارسی عنوان
استراتژی های محاسباتی برای طراحی توابع آنزیمی جدید
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
In this contribution, recent developments in the design of biocatalysts are reviewed with particular emphasis in the de novo strategy. Studies based on three different reactions, Kemp elimination, Diels-Alder and Retro-Aldolase, are used to illustrate different success achieved during the last years. Finally, a section is devoted to the particular case of designed metalloenzymes. As a general conclusion, the interplay between new and more sophisticated engineering protocols and computational methods, based on molecular dynamics simulations with Quantum Mechanics/Molecular Mechanics potentials and fully flexible models, seems to constitute the bed rock for present and future successful design strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 582, 15 September 2015, Pages 68-79
نویسندگان
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