Crystal structure of cytochrome P450 CYP105N1 from Streptomyces coelicolor, an oxidase in the coelibactin siderophore biosynthetic pathway

► The crystal structure of Streptomyces coelicolor CYP105N1 was determined at 2.9 Å resolution. ► The CYP105N1 substrate binding pocket has a wide open conformation. ► Recombinant CYP105N1 successfully converted estradiol to estriol.