Glyceraldehyde-3-phosphate dehydrogenase regulates cyclooxygenase-2 expression by targeting mRNA stability

► A novel function of GAPDH, a well-known glycolytic enzyme, was revealed. ► GAPDH down-regulated cyxlooxygenase-2 (COX-2) mRNA by its destabilization. ► GAPDH may bind to COX-2 ARE through NAD+ binding domain. ► Lipopolysaccharide markedly reduced nuclear GAPDH.