Characterization of DNA topoisomerase I from Mycobacterium tuberculosis: DNA cleavage and religation properties and inhibition of its activity

► Site-specific DNA binding and cleavage by Mycobacterium tuberculosis topoisomerase I. ► Distinct properties of the mutants in the toprim motif; loss of DNA relaxation activity. ► Inhibition of enzyme activity by oligonucleotides and monoclonal antibody.