Structural studies of N-terminal mutants of Connexin 32 using 1H NMR spectroscopy

► Connexin 32 mutants W3D and Y7D do not form functional channels. ► 1H NMR structures show alterations in the flexible turn around G12. ► Altered turn around G12 was reported previously in nonfunctional G12S and G12Y mutants. ► Connexin mutants G12S, G12Y, W3D and Y7D are not membrane inserted. ► Loss of turn around G12 may target misfolded Connexins for degradation.