Mutation of Phe413 to Tyr in catalase KatE from Escherichia coli leads to side chain damage and main chain cleavage

► The mutation in variant F413Y of KatE introduces a new reactive center. ► Main chain cleavage at R111 and T115 results. ► The side chains of Arg111, Thr115 and Tyr413 are modified. ► Catalytic turnover is required for the reactions to occur.