Intramolecular cross-linking in the native JHBP molecule

► JHBP carries juvenile hormone signal to target tissues. ► Although JHBP does not contain Trp residues it exhibits fluorescence at 420 nm. ► The unusual fluorescence of JHBP was due to the formation of dityrosine residues. ► ROS-mediated oxidation involves in vivo generation of cross-linked JHBP monomers. ► The dityrosine bridge occurs between the Y128 and Y130 residues.