Biphasic modulation of fatty acid synthase by hydrogen peroxide in Saccharomyces cerevisiae

► In a bolus addition, the initial dose of added H2O2 is rapidly consumed by cells. ► In a steady-state delivery, H2O2 concentration is constant and known. ► Fatty acid synthase regulation by H2O2 is biphasic on a narrow concentration range. ► Control of H2O2 exposure is important for cellular redox regulation studies.