Heterologous expression and characterization of the sterol 14α-demethylase CYP51F1 from Candida albicans

► CYP51F1 from C. albicans successfully converted lanosterol to its demethylated product. ► The azole antifungal agents bound tightly to CYP51F1. ► The azole Kd values of the purified mutants identified in clinical isolates were little altered.