A new insight into the role of rat cytochrome P450 24A1 in metabolism of selective analogs of 1α,25-dihydroxyvitamin D3

► Metabolism of selective vitamin D3 analogs by rat cytochrome P450 24A1 (CYP24A1). ► Docking analysis of vitamin D3 analogs using crystal structure of CYP24A1. ► Vitamin D3 analogs require discrete recognition cues to trigger CYP24A1 activity.