Expression of ZO1, vimentin, pan-cadherin and AGTR1 in tanycyte-like cells of the sulcus medianus organum

Figure 4. A, B, C, D: Immunofluorescence labeling of pan cadherin (green) at the free surface of vimentin (red)-AGTR1 (golden yellow) co-labeled tanycyte-like cells in coronal sections of rat brain at the region of caudal part of the median sulcus. Sections were also labeled with DAPI nuclear staining (blue). Deep parenchymal extension of tanycyte-like cells basal processes was less than that observed in the region of SMO. Cells labeled with AGTR1 were less than those seen in the SMO. 4 V: fourth ventricle. Scale bar = 20 μm. E & F: Photomicrographs demonstrating vimentin (red) and pan cadherin (green) labeled tanycyte-like cells in coronal sections of rat brain ME (E) and AP (F) with DAPI nuclear staining (blue). Tanycyte-like cell processes (arrow heads) reach adjacent blood vessels (BV) in the ME and pass in the parenchyma of the AP. Pan cadherin labeling gives a honeycomb appearance between tanycyte-like cells surfaces. 4 V: fourth ventricle. MO: Medulla Oblongata. Scale bar = 20 μm.373