کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8292590 | 1536734 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cystathionine gamma lyase-H2S contributes to osteoclastogenesis during bone remodeling induced by mechanical loading
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Hydrogen sulfide (H2S), a gaseous signaling molecule produced by cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), influences bone remodeling in many ways. Osteoclasts play an important role in bone remodeling during tooth movement induced by mechanical loading, which increases the rate of tooth movement. Recently, we found that osteoclasts could produce H2S. However, whether H2S modulates bone remodeling by affecting osteoclasts remains unclear. In this study, we found that CSE-H2S was a dominant H2S generating system in osteoclasts, while CBS did not generate H2S. A significant increase in CSE mRNA expression and H2S production was observed in periodontal ligament (PDL) tissues of wild-type (WT) mice after 3 days of mechanical loading. CSE gene knockout led to a significant reduction in the number of maxillary osteoclasts and in the amount of tooth movement. The number of RANKL-induced TRAP-positive osteoclasts and the mRNA expression of osteoclast markers were downregulated after 6 days of incubation in monocytes extracted from CSEâ/â mice. The expression of IL-1, IL-6 and TNF-α, which can stimulate osteoclastogenesis in periodontal tissue and serum samples, was lower in CSEâ/â mice after mechanical loading. Application of the H2S donor GYY4137 increased the number of RANKL-induced osteoclasts, the number of osteoclasts in periodontal tissues and tooth movement distance in CSEâ/â mice. The results suggested that endogenous H2S and CSE play vital roles in the osteoclastogenesis and alveolar bone resorption induced by mechanical loading.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 501, Issue 2, 22 June 2018, Pages 471-477
Journal: Biochemical and Biophysical Research Communications - Volume 501, Issue 2, 22 June 2018, Pages 471-477
نویسندگان
Shenzheng Mo, Yongmei Hua,