کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8293175 | 1536742 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-126-5p targets Malate Dehydrogenase 1 in non-small cell lung carcinomas
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Malate Dehydrogenase (MDH) 1 has recently been shown to be highly expressed and display prognostic value in non-small cell lung carcinomas (NSCLCs). However, it is not known how MDH1 expression is regulated and there is no current molecular or chemical strategy that specifically targets MDH1. This may be due to structural and enzymatic similarities with its isoenzyme, malate dehydrogenase 2 (MDH2). However, MDH1 and MDH2 are encoded by distinct genes and this opens up the possibility for modulation at the expression level. Here, we screened in silico for microRNAs (miRs) that selectively targets the 3â²UTR region of MDH1. These analyses revealed that mir-126-5p has three binding sites in the 3â²UTR region of MDH1. Additionally, we show that expression of miR-126-5p suppresses the enzymatic activity of MDH1, mitochondrial respiration and caused cell death in NSCLC cell lines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 499, Issue 2, 5 May 2018, Pages 314-320
Journal: Biochemical and Biophysical Research Communications - Volume 499, Issue 2, 5 May 2018, Pages 314-320
نویسندگان
Andre Lima Queiroz, Boxi Zhang, Dawn E. Comstock, Yuqing Hao, Matilda Eriksson, Per Hydbring, Helin Vakifahmetoglu-Norberg, Erik Norberg,