کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8293257 | 1536744 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure of a [NiFe] hydrogenase maturation protease HycI provides insights into its substrate selectivity
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Structure of a [NiFe] hydrogenase maturation protease HycI provides insights into its substrate selectivity Structure of a [NiFe] hydrogenase maturation protease HycI provides insights into its substrate selectivity](/preview/png/8293257.png)
چکیده انگلیسی
The immature large subunit of [NiFe] hydrogenases undergoes C-terminal cleavage by a specific protease in the final step of the post-translational process before assembly with other subunits. It has been reported that the [NiFe] hydrogenase maturation protease HycI from Thermococcus kodakarensis (TkHycI) has the catalytic ability to target the membrane-bound hydrogenase large subunit MbhL from T. kodakarensis. However, the detailed mechanism of its substrate recognition remains elusive. We determined the crystal structure of TkHycI at 1.59â¯Ã
resolution to clarify how TkHycI recognizes its own substrate MbhL. Although the overall structure of TkHycI is similar to that of its homologous protease TkHybD, TkHycI adopts a larger loop than TkHybD, thereby creating a broad and deep cleft. We analyzed the structural properties of the TkHycI cleft probably involved in its substrate recognition. Our findings provide novel and profound insights into the substrate selectivity of TkHycI.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 498, Issue 4, 15 April 2018, Pages 782-788
Journal: Biochemical and Biophysical Research Communications - Volume 498, Issue 4, 15 April 2018, Pages 782-788
نویسندگان
Sunghark Kwon, Yuichi Nishitani, Yoshinori Hirao, Tamotsu Kanai, Haruyuki Atomi, Kunio Miki,