کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8294891 | 1536754 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Drastic stability change of X-X mismatch in d(CXG) trinucleotide repeat disorders under molecular crowding condition
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Drastic stability change of X-X mismatch in d(CXG) trinucleotide repeat disorders under molecular crowding condition Drastic stability change of X-X mismatch in d(CXG) trinucleotide repeat disorders under molecular crowding condition](/preview/png/8294891.png)
چکیده انگلیسی
The trinucleotide repeat d(CXG) (Xâ¯=â¯A, C, G or T) is the most common sequence causing repeat expansion disorders. The formation of non-canonical structures, such as hairpin structures with X-X mismatches, has been proposed to affect gene expression and regulation, which are important in pathological studies of these devastating neurological diseases. However, little information is available regarding the thermodynamics of the repeat sequence under crowded cellular conditions where many non-canonical structures such as G-quadruplexes are highly stabilized, while duplexes are destabilised. In this study, we investigated the different stabilities of X-X mismatches in the context of internal d(CXG) self-complementary sequences in an environment with a high concentration of cosolutes to mimic the crowding conditions in cells. The stabilities of full-matched duplexes and duplexes with A-A, G-G, and T-T mismatched base pairs under molecular crowding conditions were notably decreased compared to under dilute conditions. However, the stability of the DNA duplex with a C-C mismatch base pair was only slightly destabilised. Investigating different stabilities of X-X mismatches in d(CXG) sequences is important for improving our understanding of the formation and transition of multiple non-canonical structures in trinucleotide repeat diseases, and may provide insights for pathological studies and drug development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 2, 5 February 2018, Pages 601-607
Journal: Biochemical and Biophysical Research Communications - Volume 496, Issue 2, 5 February 2018, Pages 601-607
نویسندگان
Ye Teng, Smritimoy Pramanik, Hisae Tateishi-Karimata, Tatsuya Ohyama, Naoki Sugimoto,