کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8295377 1536759 2018 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biophysical characterization of the basic cluster in the transcription repression domain of human MeCP2 with AT-rich DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Biophysical characterization of the basic cluster in the transcription repression domain of human MeCP2 with AT-rich DNA
چکیده انگلیسی
MeCP2 is a chromatin associated protein which is highly expressed in brain and relevant with Rett syndrome (RTT). There are AT-hook motifs in MeCP2 which can bind with AT-rich DNA, suggesting a role in chromatin binding. Here, we report the identification and characterization of another AT-rich DNA binding motif (residues 295 to 313) from the C-terminal transcription repression domain of MeCP2 by nuclear magnetic resonance (NMR) and isothermal calorimetry (ITC). This motif shows a micromolar affinity to AT-rich DNA, and it binds to the minor groove of DNA like AT-hook motifs. Together with the previous studies, our results provide an insight into a critical role of this motif in chromatin structure and function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 495, Issue 1, 1 January 2018, Pages 145-150
نویسندگان
, , , , , , , ,