کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8295824 | 1536761 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MeWRKY20 and its interacting and activating autophagy-related protein 8 (MeATG8) regulate plant disease resistance in cassava
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کلمات کلیدی
Cassava (Manihot esculenta)LUCNCBIGFPcoimmunoprecipitationATGMDCBiFCCDD - CSDAutophagy - اتوفاژیVIGS - ایدزchromatin immunoprecipitation - ایمن سازی کروماتینProtein-protein interaction - تعامل پروتئین-پروتئینbimolecular fluorescence complementation - تکمیل فلورسانس دو طرفه حرکتیTair - سهTranscription factor - عامل رونویسیluciferase - لوسیفرازNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیMonodansylcadaverine - مونودانسیل کاداورینCoIP - همکاری کنیدvirus-induced gene silencing - ویروس ناشی از خاموش شدن ژنconserved domain database - پایگاه داده حوزه حفظ شدهgreen fluorescent protein - پروتئین فلورسنت سبزCHiP - چیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
As a highly conserved mechanism, autophagy is responsible for the transport of cytoplasmic constituents in the vacuoles or lysosomes. Moreover, autophagy is essential for plant development and various stress responses. In this study, 34 MeATGs were systematically identified in cassava, and their transcripts were commonly regulated by Xanthomonas axonopodis pv manihotis (Xam). Through transient expression in Nicotiana benthamiana, the subcellular locations of 4 MeATG8s were revealed. Notably, MeWRKY20 was identified as physical interacting protein of MeATG8a/8f/8h and upstream transcriptional activator of MeATG8a. Through virus-induced gene silencing (VIGS) in cassava, we found that MeATG8-silenced and MeWRKY20-silenced plants resulted in disease sensitive, with less callose depositions and lower autophagic activity. This study may facilitate our understanding of the upstream MeWRKY20 and underlying target as well as interacting proteins of MeATG8s in immune response. Taken together, MeWRKY20 and MeATG8a/8f/8h are essential for disease resistance against bacterial blight by forming various transcriptional modules and interacting complex in cassava.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 494, Issues 1â2, 9 December 2017, Pages 20-26
Journal: Biochemical and Biophysical Research Communications - Volume 494, Issues 1â2, 9 December 2017, Pages 20-26
نویسندگان
Yu Yan, Peng Wang, Chaozu He, Haitao Shi,