Pyruvate diminishes the cytotoxic activity of ascorbic acid in several tumor cell lines in vitro

In summary, pyruvate diminishes the cytotoxic activity of ascorbic acid in vitro. The AFR concentration measured remains unaffected by pyruvate whereas the H2O2 concentration is reduced; confirming that pyruvate directly reacts with AA-induced H2O2, without influencing its formation. However, further experiments are needed to elucidate the complex mechanisms being responsible for the reduced efficacy of AA in vivo.