کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8296218 | 1536764 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding Human procaspase-2 phosphorylation at both S139 and S164 is required for 14-3-3 binding](/preview/png/8296218.png)
چکیده انگلیسی
Procaspase-2 phosphorylation at several residues prevents its activation and blocks apoptosis. This process involves procaspase-2 phosphorylation at S164 and its binding to the scaffolding protein 14-3-3. However, bioinformatics analysis has suggested that a second phosphoserine-containing motif may also be required for 14-3-3 binding. In this study, we show that human procaspase-2 interaction with 14-3-3 is governed by phosphorylation at both S139 and S164. Using biochemical and biophysical approaches, we show that doubly phosphorylated procaspase-2 and 14-3-3 form an equimolar complex with a dissociation constant in the nanomolar range. Furthermore, our data indicate that other regions of procaspase-2, in addition to phosphorylation motifs, may be involved in the interaction with 14-3-3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 493, Issue 2, 18 November 2017, Pages 940-945
Journal: Biochemical and Biophysical Research Communications - Volume 493, Issue 2, 18 November 2017, Pages 940-945
نویسندگان
Dana Kalabova, Aneta Smidova, Olivia Petrvalska, Miroslava Alblova, Dalibor Kosek, Petr Man, Tomas Obsil, Veronika Obsilova,