کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8297041 | 1536773 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stem cell factor (SCF) protects osteoblasts from oxidative stress through activating c-Kit-Akt signaling
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Osteoblasts regulate bone formation and remodeling, and are main target cells of oxidative stress in the progression of osteonecrosis. The stem cell factor (SCF)-c-Kit pathway plays important roles in the proliferation, differentiation and survival in a range of cell types, but little is known about its functions in osteoblasts. In this study, we found that c-Kit is functionally expressed in both osteoblastic-like MC3T3-E1 cells and primary murine osteoblasts. Its ligand SCF exerted significant cyto-protective effects against hydrogen peroxide (H2O2). SCF activated its receptor c-Kit in osteoblasts, which was required for its cyto-protective effects against H2O2. Pharmacological inhibition (by Imatinib and Dasatinib) or shRNA-mediated knockdown of c-Kit thus inhibited SCF-mediated osteoblast protection. Further investigations showed that protection by SCF against H2O2 was mediated via activation of c-Kit-dependent Akt pathway. Inhibition of Akt activation, through pharmacological or genetic means, suppressed SCF-mediated anti-H2O2 activity in osteoblasts. In summary, we have identified a new SCF-c-Kit-Akt physiologic pathway that protects osteoblasts from H2O2-induced damages, and might minimize the risk of osteonecrosis caused by oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 455, Issues 3â4, 12 December 2014, Pages 256-261
Journal: Biochemical and Biophysical Research Communications - Volume 455, Issues 3â4, 12 December 2014, Pages 256-261
نویسندگان
Lei Yang, Zhong Wu, Gang Yin, Haifeng Liu, Xiaojun Guan, Xiaoqiang Zhao, Jianguang Wang, Jianguo Zhu,