کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8297086 | 1536773 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Splicing factor SR34b mutation reduces cadmium tolerance in Arabidopsis by regulating iron-regulated transporter 1 gene
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کلمات کلیدی
ActDCHXSIETIRT1Arabidopsis - آرابیدوپسیس یا رشادیmRNA splicing - اسپری کردن mRNAactinomycin D - اکتینومایسین Dcycloheximide - سیکلوهایسیمیدinductively coupled plasma-mass spectrometry - طیف سنجی جرم پلاسما به صورت القاییICP-MS - طیفسنجی جرمی پلاسمای جفتشده القاییSplicing factor - عامل فشرده سازیSR proteins - پروتئین SRserine/arginine-rich proteins - پروتئین غنی از سریین / آرژینینCadmium - کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Serine/arginine-rich (SR) proteins are important splicing factors. However, the biological functions of plant SR proteins remain unclear especially in abiotic stresses. Cadmium (Cd) is a non-essential element that negatively affects plant growth and development. In this study, we provided clear evidence for SR gene involved in Cd tolerance in planta. Systemic expression analysis of 17 Arabidopsis SR genes revealed that SR34b is the only SR gene upregulated by Cd, suggesting its potential roles in Arabidopsis Cd tolerance. Consistent with this, a SR34b T-DNA insertion mutant (sr34b) was moderately sensitive to Cd, which had higher Cd2+ uptake rate and accumulated Cd in greater amounts than wild-type. This was due to the altered expression of iron-regulated transporter 1 (IRT1) gene in sr34b mutant. Under normal growth conditions, IRT1 mRNAs highly accumulated in sr34b mutant, which was a result of increased stability of IRT1 mRNA. Under Cd stress, however, sr34b mutant plants had a splicing defect in IRT1 gene, thus reducing the IRT1 mRNA accumulation. Despite of this, sr34b mutant plants still constitutively expressed IRT1 proteins under Cd stress, thereby resulting in Cd stress-sensitive phenotype. We therefore propose the essential roles of SR34b in posttranscriptional regulation of IRT1 expression and identify it as a regulator of Arabidopsis Cd tolerance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 455, Issues 3â4, 12 December 2014, Pages 312-317
Journal: Biochemical and Biophysical Research Communications - Volume 455, Issues 3â4, 12 December 2014, Pages 312-317
نویسندگان
Wentao Zhang, Bojing Du, Di Liu, Xiaoting Qi,