کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8297227 | 1536775 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Unconventional secretion of the mutated 19Â kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Unconventional secretion of the mutated 19Â kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells Unconventional secretion of the mutated 19Â kDa protein of Oplophorus luciferase (nanoKAZ) in mammalian cells](/preview/png/8297227.png)
چکیده انگلیسی
The putative amino-terminal signal peptide of the catalytic 19Â kDa protein (KAZ) of Oplophorus luciferase was found to be a functional secretory peptide in mammalian cells. A 16 amino acid substituted mutant of KAZ (nanoKAZ) could be secreted from mammalian cells using the amino-terminal signal peptide of KAZ, but KAZ could not be secreted at all. Notably, nanoKAZ lacking the amino-terminal signal peptide could be secreted from mammalian cells, and the distribution of nanoKAZ on the cell membrane was confirmed by video-rate bioluminescence imaging. Thus, nanoKAZ lacking the amino-terminal signal peptide was expressed in the cytoplasm, translocated to the cell membrane, and released into the culture medium through an endoplasmic reticulum-Golgi-independent pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 4, 8 August 2014, Pages 1313-1319
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 4, 8 August 2014, Pages 1313-1319
نویسندگان
Satoshi Inouye, Jun-ichi Sato, Yuiko Sahara-Miura, Takamitsu Hosoya, Takahiro Suzuki,