کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8297508 | 1536777 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Large changes in NAD levels associated with CD38 expression during HL-60 cell differentiation
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
NAD is an important cofactor involved in multiple metabolic reactions and as a substrate for several NAD-dependent signalling enzymes. One such enzyme is CD38 which, alongside synthesising Ca2+-releasing second messengers and acting as a cell surface receptor, has also been suggested to play a key role in NAD+ homeostasis. CD38 is well known as a negative prognostic marker in B-CLL but the role of its enzymatic activity has not been studied in depth to date. We have exploited the HL-60 cell line as a model of inducible CD38 expression, to investigate CD38-mediated regulation intracellular NAD+ levels and the consequences of changes in NAD+ levels on cell physiology. Intracellular NAD+ levels fell with increasing CD38 expression and this was reversed with the CD38 inhibitor, kuromanin confirming the key role of CD38 in NAD+ homeostasis. We also measured the consequences of CD38 expression during the differentiation on a number of functions linked to NAD+ and we show that some but not all NAD+-dependent processes are significantly affected by the lowered NAD+ levels. These data suggest that both functional roles of CD38 might be important in the pathogenesis of B-CLL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 442, Issues 1â2, 6 December 2013, Pages 51-55
Journal: Biochemical and Biophysical Research Communications - Volume 442, Issues 1â2, 6 December 2013, Pages 51-55
نویسندگان
Zainab N. Al-Abady, Barbara Durante, A. John Moody, Richard A. Billington,