p38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells

► The effect of 25-HC on colon cancer cells was very similar to those of anicequol. ► p38MAPK was involved in anicequol- and 25-HC-induced anoikis in DLD-1 cells. ► ROCK was also associated with anoikis induced by anicequol or 25-HC. ► p38MAPK and ROCK shared the same anoikis induction pathway.