Structure of the complex of Neisseria gonorrhoeae N-acetyl-l-glutamate synthase with a bound bisubstrate analog

► Preparation of a bisubstrate analog CoA-NAG using enzyme itself as the catalyst. ► Determination of the structure of the complex of ngNAGS bound with CoA-NAG. ► Mutagenesis and kinetic studies of active site residues. ► Catalytic mechanism for ngNAGS enzymatic reaction.