The human Ino80 binds to microtubule via the E-hook of tubulin: Implications for the role in spindle assembly

► The N-terminal domain of hIno80 is important for binding to the spindle. ► The hIno80 N-terminal domain binds to tubulin and microtubule in vitro. ► The E-hook of tubulin is critical for hIno80 binding to tubulin and microtubule. ► Tip49a does not bind to microtubule and dispensable for spindle formation.