کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8299196 | 1537052 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impaired proton pumping in cytochrome c oxidase upon structural alteration of the D pathway
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CytcOn-SideP-sidepositive side of the membraneelectron transfer - انتقال الکترونProton transfer - انتقال پروتونRedox - ردوکس(اکسایش و کاهش)cytochrome c oxidase - سیتوکروم سی اکسیدازnegative side of the membrane - طرف منفی غشاءHeme-copper oxidase - هیدروژن اکسیداز مسMembrane protein - پروتئین غشائیProton pump - پمپ پروتون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cytochrome c oxidase is a membrane-bound enzyme, which catalyses the one-electron oxidation of four molecules of cytochrome c and the four-electron reduction of O2 to water. Electron transfer through the enzyme is coupled to proton pumping across the membrane. Protons that are pumped as well as those that are used for O2 reduction are transferred though a specific intraprotein (D) pathway. Results from earlier studies have shown that replacement of residue Asn139 by an Asp, at the beginning of the D pathway, results in blocking proton pumping without slowing uptake of substrate protons used for O2 reduction. Furthermore, introduction of the acidic residue results in an increase of the apparent pKa of E286, an internal proton donor to the catalytic site, from 9.4 to ~Â 11. In this study we have investigated intramolecular electron and proton transfer in a mutant cytochrome c oxidase in which a neutral residue, Thr, was introduced at the 139 site. The mutation results in uncoupling of proton pumping from O2 reduction, but a decrease in the apparent pKa of E286 from 9.4 to 7.6. The data provide insights into the mechanism by which cytochrome c oxidase pumps protons and the structural elements involved in this process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1777, Issues 7â8, JulyâAugust 2008, Pages 897-903
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1777, Issues 7â8, JulyâAugust 2008, Pages 897-903
نویسندگان
HÃ¥kan Lepp, Lina Salomonsson, Jia-Peng Zhu, Robert B. Gennis, Peter Brzezinski,