کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8299313 1537129 2018 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calcium accelerates SNARE-mediated lipid mixing through modulating α-synuclein membrane interaction
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Calcium accelerates SNARE-mediated lipid mixing through modulating α-synuclein membrane interaction
چکیده انگلیسی
α-Synuclein is involved in Parkinson's disease, and its interaction with cell membrane is vital to its pathological and physiological functions. We have shown that Ca2+ can regulate α-synuclein membrane interaction, but the physiological role of Ca2+ in modulating α-synuclein membrane interaction is still unexplored. Based on the previous findings that α-synuclein inhibits membrane fusion and its inhibitory effect is highly related to its membrane binding, here we employed solution state Nuclear Magnetic Resonance (NMR) spectroscopy and the ensemble fluorescence fusion assay to show that Ca2+ can modulate the inhibitory effect of α-synuclein on SNARE-mediated membrane fusion through disrupting α-synuclein membrane interaction, resulting in acceleration of SNARE-mediated membrane fusion. These results suggest a modulatory effect of Ca2+ on membrane mediated normal function of α-synuclein, which of importance for the study of the Parkinson's disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1860, Issue 9, September 2018, Pages 1848-1853
نویسندگان
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