Interaction of local anesthetics with lipid bilayers investigated by 1H MAS NMR spectroscopy

► Membrane location of 4 local anesthetics was studied by quantitative 1H MAS NMR. ► Aromatic rings of all local anesthetics are located in the lipid‐water interface. ► Small difference in the membrane location originate from molecular structures. ► Hydrophobic side chains of dibucaine and tetracaine are inserted into the membrane.