Electrophysiological properties of heteromeric TRPV4-C1 channels

► The predominate assembly type of heteromeric TRPV4-C1 channels is 2V4:2C1. ► Properties of TRPV4-C1 channels were examined at single-channel and whole-cell level. ► Monovalent cation permeability profiles of TRPV4-C1 were fit to Eisenman sequence VI. ► Heteromeric TRPV4-C1 channels were slightly more permeable to Ca2+. ► TRPV4-C1 channels had a reduced sensitivity to extracellular Ca2+ inhibition.