Photo-dynamic induction of oxidative stress within cholesterol-containing membranes: Shape transitions and permeabilization

► The cholesterol content of membranes influences their photo-induced permeabilization. ► Permeabilization efficiency is decreased by cholesterol-induced stress relaxation. ► We report a direct observation of the dynamics response of biomimetic vesicles. ► We propose the first quantitative analysis and physico-chemical interpretation. ► The role of cholesterol in membrane dynamics and permeabilization is clarified.