کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8300870 1537475 2018 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dysfunction of pulmonary surfactant mediated by phospholipid oxidation is cholesterol-dependent
ترجمه فارسی عنوان
اختلال در سورفاکتانت ریوی ناشی از اکسیداسیون فسفولیپید وابسته به کلسترول است
کلمات کلیدی
سورفاکتانت ریوی، اکسیداسیون، کلسترول، اختلال عملکرد فسفولیپید ها، رادیکالهای واکنشی،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Pulmonary surfactant forms a cohesive film at the alveolar air-lung interface, lowering surface tension, and thus reducing the work of breathing and preventing atelectasis. Surfactant function becomes impaired during inflammation due to degradation of the surfactant lipids and proteins by free radicals. In this study, we examine the role of reactive nitrogen (RNS) and oxygen (ROS) species on surfactant function with and without physiological cholesterol levels (5-10%). Surface activity was assessed in vitro in a captive bubble surfactometer (CBS). Surfactant chemistry, monolayer fluidity and thermodynamic behavior were also recorded before and after oxidation. We report that physiologic amounts of cholesterol combined with oxidation results in severe impairment of surfactant function. We also show that surfactant polyunsaturated phospholipids are the most susceptible to oxidative alteration. Membrane thermodynamic experiments showed significant surfactant film stiffening after free radical exposure in the presence of cholesterol. These results point to a previously unappreciated role for cholesterol in amplifying defects in surface activity caused by oxidation of pulmonary surfactant, a finding that may have implications for treating several lung diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1862, Issue 4, April 2018, Pages 1040-1049
نویسندگان
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