کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8300878 | 1537476 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The mouse Gm853 gene encodes a novel enzyme: Leucine decarboxylase
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کلمات کلیدی
HEKAntizymePLPLDCDMEMOrnithine decarboxylaseα-DifluoromethylornithineDFMOODCDulbecco's modified Eagle Medium - Eagle Medium اصلاح شده Dulbeccotestosterone - تستوسترونProtein evolution - تکامل پروتئینAntizyme inhibitor - مهارکننده آنتی ژیPyridoxal phosphate - پرییدکسال فسفاتPolyamine - پلی آمینEnzyme catalysis - کاتالیزور آنزیمhuman embryonic kidney - کلیه جنین انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The mouse Gm853 gene encodes a novel enzyme: Leucine decarboxylase The mouse Gm853 gene encodes a novel enzyme: Leucine decarboxylase](/preview/png/8300878.png)
چکیده انگلیسی
Ornithine decarboxylase (ODC) is a key enzyme in the biosynthesis of polyamines. ODC-antizyme inhibitors (AZINs) are homologous proteins of ODC, devoid of enzymatic activity but acting as regulators of polyamine levels. The last paralogue gene recently incorporated into the ODC/AZINs family is the murine Gm853, which is located in the same chromosome as AZIN2, and whose biochemical function is still unknown. By means of transfection assays of HEK293T cells with a plasmid containing the coding region of Gm853, we show here that unlike ODC, GM853 was a stable protein that was not able to decarboxylate l-ornithine or l-lysine and that did not act as an antizyme inhibitor. However, GM853 showed leucine decarboxylase activity, an enzymatic activity never described in animal cells, and by acting on l-leucine (Km = 7.03 Ã 10â 3 M) it produced isopentylamine, an aliphatic monoamine with unknown function. The other physiological branched-chain amino acids, l-valine and l-isoleucine were poor substrates of the enzyme. Gm853 expression was mainly detected in the kidney, and as Odc, it was stimulated by testosterone. The conservation of Gm853 orthologues in different mammalian species, including primates, underlines the possible biological significance of this new enzyme. In this study, we describe for the first time a mammalian enzyme with leucine decarboxylase activity, therefore proposing that the gene Gm853 and its protein product should be named as leucine decarboxylase (Ldc, LDC).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1862, Issue 3, March 2018, Pages 365-376
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1862, Issue 3, March 2018, Pages 365-376
نویسندگان
Ana Lambertos, Bruno Ramos-Molina, David Cerezo, Andrés J. López-Contreras, Rafael Peñafiel,