کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8301304 | 1537676 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lpaatδ/Agpat4 deficiency impairs maximal force contractility in soleus and alters fibre type in extensor digitorum longus muscle
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کلمات کلیدی
AGPATGPATLPAATPDHDGKLPAEDLMnSODETsmTORTLCOXPHOSphosphatidic acid - اسید فسفاتیدیکextensor digitorum longus - اکستانسور بلند شست انگشتانtriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولelectron transport chain - زنجیره انتقال الکترونMyosin heavy chain - زنجیره سنگین میوزینmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزElectron transport system - سیستم حمل و نقل الکترونیphosphatidylinositol - فسفاتیدیل اینوزیتولphosphatidylcholine - فسفاتیدیل کولینOxidative phosphorylation - فسفوریلاسیون اکسیداتیوlysophosphatidic acid acyltransferase - لیسفسفید فسفیدید اسید آتیل ترانسفرازMHC - مجموعه سازگاری بافتی اصلیMechanistic target of rapamycin - هدف مکانیکی رپامایسینETc - و غیرهpyruvate dehydrogenase - پیرووات دهیدروژنازCardiolipin - کاردیولیپینSUIT - کت و شلوارthin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lysophosphatidic acid acyltransferase (LPAAT) δ/acylglycerophosphate acyltransferase 4 is a mitochondrial enzyme and one of five homologues that catalyze the acyl-CoA-dependent synthesis of phosphatidic acid (PA) from lysophosphatidic acid. We studied skeletal muscle LPAATδ and found highest levels in soleus, a red oxidative fibre-type that is rich in mitochondria, and lower levels in extensor digitorum longus (EDL) (white glycolytic) and gastrocnemius (mixed fibre-type). Using Lpaatδ-deficient mice, we found no change in soleus or EDL mass, or in treadmill time-to-exhaustion compared to wildtype littermates. There was, however, a significant reduction in the proportion of type I and type IIA fibres in EDL but, surprisingly, not soleus, where these fibre-types predominate. Also unexpectedly, there was no impairment in force generation by EDL, but a significant reduction by soleus. Oxidative phosphorylation and activity of complexes I, Iâ¯+â¯II, III, and IV in soleus mitochondria was unchanged and therefore could not explain this effect. However, pyruvate dehydrogenase activity was significantly reduced in Lpaatδâ/â soleus and EDL. Analysis of cellular lipids indicated no difference in soleus triacylglycerol, but specific elevations in soleus PA and phosphatidylethanolamine levels, likely due to a compensatory upregulation of Lpaatβ and Lpaatε in Lpaatδâ/â mice. An anabolic effect for PA as an activator of skeletal muscle mTOR has been reported, but we found no change in serine 2448 phosphorylation, indicating reduced soleus force generation is unlikely due to the loss of mTOR activation by a specific pool of LPAATδ-derived PA. Our results identify an important role for LPAATδ in soleus and EDL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1863, Issue 7, July 2018, Pages 700-711
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1863, Issue 7, July 2018, Pages 700-711
نویسندگان
Ryan M. Bradley, Darin Bloemberg, Juan J. Aristizabal Henao, Ashkan Hashemi, Andrew S. Mitchell, Val A. Fajardo, Catherine Bellissimo, Emily B. Mardian, Eric Bombardier, Marie-France Paré, Katherine A. Moes, Ken D. Stark, A. Russell Tupling,