کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8301828 1537713 2015 29 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short-chain polyisoprenoids in the yeast Saccharomyces cerevisiae - New companions of the old guys
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Short-chain polyisoprenoids in the yeast Saccharomyces cerevisiae - New companions of the old guys
چکیده انگلیسی
Dolichols are, among others, obligatory cofactors of protein glycosylation in eukaryotic cells. It is well known that yeast cells accumulate a family of dolichols with Dol-15/16 dominating while upon certain physiological conditions a second family with Dol-21 dominating is noted. In this report we identified the presence of additional short-chain length polyprenols - all-trans Pren-7 in three yeast strains (SS328, BY4741 and L5366), Pren-7 was accompanied by traces of putative Pren-6 and -8. Moreover, in two of these strains a single polyprenol mainly-cis-Pren-11 was synthesized at the stationary phase of growth. Identity of polyprenols was confirmed by HR-HPLC/MS, NMR and metabolic labeling. Additionally, simvastatin inhibited their biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 10, October 2015, Pages 1296-1303
نویسندگان
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