کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8302306 | 1537726 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cyclic phosphatidic acid and lysophosphatidic acid induce hyaluronic acid synthesis via CREB transcription factor regulation in human skin fibroblasts
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کلمات کلیدی
CPAPTXERKLPAHASSEAPmRNACMF-PBSLPARCREPBSCREBPI3KSTAT3BSA - BSACRE binding protein - CRE پروتئین اتصالNFκB - NFKBmessenger RNA - RNA messengerbovine serum albumin - آلبومین سرم گاوSecreted alkaline phosphatase - آلکالن فسفاتاز تثبیت شدهcyclic phosphatidic acid - اسید فسفاتیک cycliclysophosphatidic acid - اسید لیسفسفیدیدHyaluronic acid - اسید هیالورونیکchromatin immunoprecipitation - ایمن سازی کروماتینpertussis toxin - سموم سورافنیnuclear factor κB - فاکتور هسته ای κBphosphatidylinositol-3 kinase - فسفاتیدیلینوزیتول 3 کینازphospho-ERK - فسفو ERKHuman skin fibroblast - فیبروبلاست پوست انسانsignal transducer and activator of transcription 3 - مبدل سیگنال و فعال کننده رونویسی 3Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHyaluronan synthase - هیالورونان سنتازpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPERK - پرکCHiP - چیپextracellular-signal-regulated kinase - کیناز تنظیم شده خارج سلولی سیگنالLPA receptor - گیرنده LPARock - یا راک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cyclic phosphatidic acid (cPA) is a naturally occurring phospholipid mediator and an analog of the growth factor-like phospholipid lysophosphatidic acid (LPA). cPA has a unique cyclic phosphate ring at the sn-2 and sn-3 positions of its glycerol backbone. We showed before that a metabolically stabilized cPA derivative, 2-carba-cPA, relieved osteoarthritis pathogenesis in vivo and induced hyaluronic acid synthesis in human osteoarthritis synoviocytes in vitro. This study focused on hyaluronic acid synthesis in human fibroblasts, which retain moisture and maintain health in the dermis. We investigated the effects of cPA and LPA on hyaluronic acid synthesis in human fibroblasts (NB1RGB cells). Using particle exclusion and enzyme-linked immunosorbent assays, we found that both cPA and LPA dose-dependently induced hyaluronic acid synthesis. We revealed that the expression of hyaluronan synthase 2 messenger RNA and protein is up-regulated by cPA and LPA treatment time dependently. We then characterized the signaling pathways up-regulating hyaluronic acid synthesis mediated by cPA and LPA in NB1RGB cells. Pharmacological inhibition and reporter gene assays revealed that the activation of the LPA receptor LPAR1, Gi/o protein, phosphatidylinositol-3 kinase (PI3K), extracellular-signal-regulated kinase (ERK), and cyclic adenosine monophosphate response element-binding protein (CREB) but not nuclear factor κB induced hyaluronic acid synthesis by the treatment with cPA and LPA in NB1RGB cells. These results demonstrate for the first time that cPA and LPA induce hyaluronic acid synthesis in human skin fibroblasts mainly through the activation of LPAR1-Gi/o followed by the PI3K, ERK, and CREB signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1841, Issue 9, September 2014, Pages 1256-1263
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1841, Issue 9, September 2014, Pages 1256-1263
نویسندگان
Katsura Maeda-Sano, Mari Gotoh, Toshiro Morohoshi, Takao Someya, Hiromu Murofushi, Kimiko Murakami-Murofushi,