کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8302603 | 1537740 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibitory effects of tannic acid on fatty acid synthase and 3T3-L1 preadipocyte
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کلمات کلیدی
FBSAc-CoAmalonyl-CoAPPARACPPBSIC50FASDMSO - DMSOMTT - MTTAcetyl CoA - استیل کولاTannic acid - اسید تانیکfatty acid synthase - اسید چرب سنتازInhibitor - بازدارندهLipid accumulation - تجمع لیپیدAdipocyte differentiation - تمایز آدپیوسیتیThioesterase - تیواستروئیدDimethyl sulfoxide - دیمتیل سولفواکسیدEnoyl reductase - ردوکتاز Enoylfetal bovine serum - سرم جنین گاوMat - ماتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریhalf-inhibitory concentration - نیمه مهار کننده غلظتacyl carrier protein - پروتئین حامل آکیلKetoacyl synthase - کتواسیل سنتازPeroxisome proliferator-activated receptors - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Tannic acid is a hydrolyzable tannin that exists in many widespread edible plants with a variety of biological activities. In this study, we found that tannic acid potently inhibited the activity of fatty acid synthase (FAS) in a concentration-dependent manner with a half-inhibitory concentration value (IC50) of 0.14 μM. The inhibition kinetic results showed that the inhibition of FAS by tannic acid was mixed competitive and noncompetitive manner with respect to acetyl-CoA and malonyl-CoA, but uncompetitive to NADPH. Tannic acid prevented the differentiation of 3T3-L1 pre-adipocytes, and thus repressed intracellular lipid accumulation. In the meantime, tannic acid decreased the expression of FAS and down-regulated the mRNA level of FAS and PPARγ during adipocyte differentiation. Further studies showed that the inhibitory effect of tannic acid did not relate to FAS non-specific sedimentation. Since FAS was believed to be a therapeutic target of obesity, these findings suggested that tannic acid was considered having potential in the prevention of obesity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1831, Issue 7, July 2013, Pages 1260-1266
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1831, Issue 7, July 2013, Pages 1260-1266
نویسندگان
Huijin Fan, Dan Wu, Weixi Tian, Xiaofeng Ma,