Tight association of N-terminal and catalytic subunits of rabbit 12/15-lipoxygenase is important for protein stability and catalytic activity

► Tight interdomain association is important for structural performance of 12/15-LOX. ► Tyr98 is involved in aromatic interaction between two subunits of 12/15-LOX. ► A loss of an aromatic interaction destabilize tertiary structure of 12/15-LOX. ► This structural alteration is associated with a functional distortion of 12/15-LOX.