Phospholipase-D activity and inflammatory response induced by brown spider dermonecrotic toxin: Endothelial cell membrane phospholipids as targets for toxicity

► Brown spider recombinant toxin hydrolyzes sphingomyelin. ► Catalytic site-directed mutagenesis H12A reduces drastically substrate hydrolysis. ► Dermonecrosis and inflammation induced by toxin depend on phospholipase activity. ► Both recombinant forms bound to lipids in blot and endothelial cell membrane. ► Phospholipase-D activity induces endothelial citotoxicity. ► Lysophospholipids and glycerophospholipids are also substrates for LiRecDT1.