کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8303481 | 1537808 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ApoA-I induces a preferential efflux of monounsaturated phosphatidylcholine and medium chain sphingomyelin species from a cellular pool distinct from HDL3 mediated phospholipid efflux
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کلمات کلیدی
DihydrosphingomyelinE-LDLAc-LDLHMDMLRPSPMHDLLCATABCAcetylated LDL - LDL آستیلت شدهapo - آپوapolipoprotein - آپولیپوپروتئینsphingomyelin - اسفنگومیلینTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیphosphatidylcholine - فسفاتیدیل کولینphosphatidylethanolamine - فسفاتیدیلتانولامینPhosphatidylserine - فسفاتیدیلسرینLecithin:cholesterol acyltransferase - لسیتین: آستیلترانسفراز کلسترولhigh density lipoprotein - لیپوپروتئین با چگالی بالاLipidomics - لیپیدومیکسhuman monocyte-derived macrophages - ماکروفاژهای حاصل از مونوسیت انسانstable isotope labeling - نشانه ایزوتوپ پایدارLDL receptor-related protein - پروتئین مرتبط با گیرنده LDLcholesteryl ester - کلسترول استرtotal cholesterol - کلسترول تامfree cholesterol - کلسترول رایگانATP-binding cassette - کیت اتصال به ATPScavenger receptor - گیرنده مخربelectrospray ionization - یونیزاسیون الکترو اسپری
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Electrospray ionization tandem mass spectrometry (ESI-MS/MS) was used for a detailed analysis of cellular phospholipid and cholesterol efflux in free cholesterol (FC) loaded human primary fibroblasts and human monocyte-derived macrophages (HMDM) loaded with enzymatically modified LDL (E-LDL). Although both cell models differed significantly in their cellular lipid composition, a higher apoA-I specific efflux was found for monounsaturated phosphatidylcholine (PC) species together with a decreased contribution of polyunsaturated PC species in both cell types. Moreover, medium chain sphingomyelin (SPM) species SPM 14:0 and SPM 16:1 were translocated preferentially to apoA-I in both cell types. In contrast to fibroblasts, HMDM displayed a considerable proportion of cholesteryl esters (CE) in basal and apoA-I specific efflux media, most likely due to secretion of CE associated to apoE. Analysis of HDL3 mediated lipid efflux from HMDM using D9-choline and 13C3-FC stable isotope labeling revealed significantly different D9-PC and D9-SPM species pattern for apoA-I and HDL3 specific efflux media, which indicates a contribution of distinct cellular phospholipid pools to apoA-I and HDL3 mediated efflux. Together with a partial loading of fibroblasts and HMDM with HDL3-derived CE species, these data add further evidence for retroendocytosis of HDL. In summary, analysis of apoA-I/ABCA1 and HDL3 mediated lipid efflux by ESI-MS/MS demonstrated a preferential efflux of monounsaturated PC and medium chain SPM to apoA-I. Moreover, this is the first study, which provides evidence for distinct cellular phospholipid pools used for lipid transfer to apoA-I and HDL3 from the analysis of phospholipid species pattern in HMDM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1771, Issue 7, July 2007, Pages 853-863
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1771, Issue 7, July 2007, Pages 853-863
نویسندگان
Rainer Schifferer, Gerhard Liebisch, Sascha Bandulik, Thomas Langmann, Ashraf Dada, Gerd Schmitz,