کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8303543 1537822 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Serum amyloid A (SAA)-induced remodeling of CSF-HDL
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Serum amyloid A (SAA)-induced remodeling of CSF-HDL
چکیده انگلیسی
Inflammation is a risk factor for Alzheimer's disease. Serum amyloid A (SAA) is an acute phase protein that dissociates apolipoprotein AI (apoAI) from plasma HDL. In cerebrospinal fluid (CSF), the SAA concentration is much higher in subjects with Alzheimer's disease than in controls. CSF-HDL is rich in apoE, which plays an important role as a ligand for lipoprotein receptors in the central nervous system (CNS). To clarify whether SAA dissociates apoE from CSF-HDL, we added recombinant SAA to CSF and determined the apoE distribution in the CSF using native two-dimensional gel electrophoresis. We found that SAA dissociated apoE from CSF-HDL in a dose-dependent manner. This effect was more evident in apoE4 carriers than in apoE3 or apoE2 carriers. After a 24-h incubation at 37 °C, SAA continuously dissociated apoE from CSF-HDL. Amyloid β (Aβ) fragments (1-42) were bound to large CSF-HDL but not to apoE dissociated by SAA. In conclusion, SAA dissociates apoE from CSF-HDL. We postulate that inflammation in the CNS may impair Aβ clearance due to the loss of apoE from CSF-HDL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1761, Issue 4, April 2006, Pages 424-433
نویسندگان
, , , , , , , , , , , ,