کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8305420 | 1538429 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hypermethylation contributes to down-regulation of lysosomal β-hexosaminidase α subunit in prostate cancer cells
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کلمات کلیدی
N-acetyl-β-D-hexosaminidaseTSAGLSHexosaminidase AHEXBHexAGSLSMugsβ-hex5-Aza-dCMTT - MTTmethylation specific PCR - PCR اختصاصی متیلاسیونTrichostatin A - تریکوستاتین Aceramide - سرامیدProstate cancer - سرطان پروستاتPromoter methylation - متیلاسیون سازندهMug - کوزهGlycosphingolipids - گلیسفینگولایپید هاGlycolipids - گلیکوالیپید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hypermethylation contributes to down-regulation of lysosomal β-hexosaminidase α subunit in prostate cancer cells Hypermethylation contributes to down-regulation of lysosomal β-hexosaminidase α subunit in prostate cancer cells](/preview/png/8305420.png)
چکیده انگلیسی
β-Hexosaminidase, involved in degradation of glycoproteins and glycosphingolipids, is altered in several tumours leading to enhanced migration capacity. To date, the expression of the β-hexosaminidase isoenzymes in prostate cancer cells has not been elucidated. By using PC3, LNCaP, DUCaP, MDAPCa 2b, and hyperplasic prostate (BPH-1) cell lines, we analysed the β-hexosaminidase activity in each cell line and determined β-hexosaminidase α subunit gene expression in PC3, LNCaP, and BPH-1. We then investigated the methylation status of the gene promoter and determined the cellular responses of PC3 and LNCaP after transfection with β-hexosaminidase α subunit. We found that each prostate cancer cell line had a decrease in total hexosaminidase activity and that the lack of hexosaminidase A activity, observed in PC3 and LNCaP cells, was associated with mRNA disappearance. The HEXA promoter region in LNCaP and PC3 cell lines had methylated CpG islands, as confirmed by 5â²-Aza-2â²-deoxycitidine treatment, in PC3 cells, used as cell cancer model. We also tested, the involvement of hexosaminidase A in the migration capacity by migration assay using Hex α subunit-transfected PC3. Finally, we found that, after Hex α subunit transfection, both PC3 and LNCaP were less susceptible to exogenous ceramide treatment. Results indicate a likely contribution of the lysosomal enzyme to the acquisition of cancerous features.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 101, June 2014, Pages 75-82
Journal: Biochimie - Volume 101, June 2014, Pages 75-82
نویسندگان
Egidia Costanzi, Lorena Urbanelli, Ilaria Bellezza, Alessandro Magini, Carla Emiliani, Alba Minelli,