کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8305485 | 1538429 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic and regulatory properties of the trehalose-6-phosphate synthase from the thermoacidophilic archaeon Thermoplasma acidophilum
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کلمات کلیدی
Thermoplasma acidophilum - Thermoplasma اسیدوفیلمTrehalose 6-phosphate - تراهلوز 6-فسفاتTrehalose-6-phosphate synthase - تراهلوز 6-فسفات سنتازTrehalose-6-phosphate phosphatase - ترهلوز 6 فسفات فسفاتازEnzyme regulation - تنظیم آنزیمMolecular modeling - مدل سازی مولکولیMechanosensitive channel - کانال مکانی حساسGlucose 6-phosphate - گلوکز 6-فسفاتADP-glucose - گلوکز ADPUDP-glucose - گلوکز UDPGlycosyltransferase - گلیکوزیلتransferase
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Trehalose-6-phosphate synthase plays an important role in trehalose metabolism. It catalyzes the transfer of glucose from UDP-glucose (UDPG) to glucose 6-phosphate to produce trehalose-6-phosphate. Herein we describe the characterization of a trehalose-6-phosphate synthase from the thermoacidophilic archaeon Thermoplasma acidophilum. The dimeric enzyme could utilize UDPG, ADP-Glucose (ADPG) and GDP-Glucose (GDPG) as glycosyl donors and various phosphorylated monosaccharides as glycosyl acceptors. The optimal temperature and pH were found to be 60 °C and pH 6, and the enzyme exhibited notable pH and thermal stability. The enzymatic activity could be stimulated by divalent metal ions and polyanions heparin and chondroitin sulfate. Moreover, the protein was considerably resistant to additives ethanol, EDTA, urea, DTT, SDS, β-mercaptoethanol, methanol, isopropanol and n-butanol. Molecular modeling and mutagenesis analysis revealed that the N-loop region was important for the catalytic efficiency of the enzyme, indicating different roles of N-loop sequences in different trehalose-6-phosphate synthases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 101, June 2014, Pages 215-220
Journal: Biochimie - Volume 101, June 2014, Pages 215-220
نویسندگان
Yanyan Gao, Ying Jiang, Qiulei Liu, Ruiming Wang, Xinli Liu, Bo Liu,