Validation of a multiplex immunoassay for serum angiogenic factors as biomarkers for aggressive prostate cancer

► Performance of the Bio-Plex Human Cancer Biomarker Panel 1 was evaluated. ► All 16 assays had good reproducibility, sensitivity, and wide dynamic ranges. ► sTIE-2 was elevated in serum of prostate cancer patients with GS of 8, 9 or 10.