Application of pyrosequencing technique for improved detection of K-Ras mutation in formalin-fixed and paraffin-embedded prostate carcinoma tissues in Chinese patients

► K-Ras mutations were evaluated by pyrosequencing in prostate cancer FFPE samples. ► We compared the pyrosequencing and Sanger sequencing for detecting mutations. ► Increasing detection of mutations in FFPE prostate cancer samples by pyrosequencing ► The K-Ras mutation was more frequent in Chinese population by pyrosequencing.