Improved analysis of C26:0-lysophosphatidylcholine in dried-blood spots via negative ion mode HPLC-ESI-MS/MS for X-linked adrenoleukodystrophy newborn screening

► We describe a rapid and simple procedure to extract lyso-PC from dried-blood spots. ► Lyso-PCs are quantitated using negative ion mode HPLC-ESI-MS/MS. ► A previously reported interfering compound (in positive ion mode) was not observed.